|
|
|
| Registros recuperados: 76 | |
|
|
| Bechemin, Christian; Soletchnik, Patrick; Polsenaere, Pierre; Le Moine, Olivier; Pernet, Fabrice; Protat, Martin; Fuhrmann, Marine; Quere, Claudie; Goulitquer, Sophie; Corporeau, Charlotte; Lapegue, Sylvie; Travers, Marie-agnes; Morga, Benjamin; Garrigues, Manon; Garcia, Celine; Haffner, Philippe; Dubreuil, Christine; Faury, Nicole; Baillon, Laury; Baud, Jean-pierre; Renault, Tristan. |
| Mass mortality outbreaks (90-100%) in both juvenile and adult blue mussels were reported in 2014 along the Pertuis Charentais area of the French Atlantic coast. A series of studies were carried out to learn more about the phenomenon through detecting pathogens, describing environmental conditions and establishing the physiological status of the animals. Bacteria identif ied as belonging to the V. splendidus species were detected in dying mussels and seem able to cause mortality in blue mussels in the laboratory. Unusual environmental conditions (large amounts of fresh water, re-suspension of sediments during a series of storms, and a high renewal rate of specific water masses in Brittany’s Pertuis area) may be partially responsible for sparking the phenomen |
| Tipo: Text |
Palavras-chave: Mortalités; Moules; Pertuis Charentais; Vibrio splendidus; Environnement; Mortality; Mussels; Pertuis Charentais; Vibrio splendidus; Environment. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00312/42343/41682.pdf |
| |
|
|
| Bechemin, Christian; Soletchnik, Patrick; Polsenaere, Pierre; Le Moine, Olivier; Pernet, Fabrice; Protat, Martin; Fuhrmann, Marine; Quere, Claudie; Goulitquer, Sophie; Corporeau, Charlotte; Renault, Tristan; Lapegue, Sylvie; Travers, Marie-agnes; Morga, Benjamin; Garriques, Manon; Garcia, Celine; Haffner, Philippe; Dubreuil, Christine; Faury, Nicole; Baillon, Laury; Baud, Jean-pierre. |
| Suite aux surmortalités observées au sein des élevages de moules touchant à la fois les animaux adultes et les juvéniles au mois de mars, dans les Pertuis Charentais, la Direction Générale de l’Alimentation (DGAl) a adressé une saisine à Ifremer le 04 avril 2014. Celle-ci fait état d’une demande d’appui scientifique et technique d’Ifremer pour tenter d’apporter des éléments de réponse par rapport à cette mortalité subite et importante. L’Ifremer a proposé un projet d’action permettant l’expertise du phénomène de surmortalité de la moule bleue, selon 3 axes : l’impact environnemental, la pathologie et les caractéristiques physiologiques des moules. Il s’agit de mettre en évidence la présence ou non d’agents infectieux connus ou émergents, d’apporter des... |
| Tipo: Text |
|
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00229/34022/32387.pdf |
| |
|
| |
|
|
| Brosset, Pablo; Fromentin, Jean-marc; Menard, Frederic; Pernet, Fabrice; Bourdeix, Jean-herve; Bigot, Jean-louis; Van Beveren, Elisabeth; Perez Rodia, Maria A.; Choy, Sandrine; Saraux, Claire. |
| As condition is a key variable in population dynamics (especially for survival, growth and reproduction), the use of well-defined and accurate fish condition indices is capital. In particular, condition indices (morphometric, bioenergetic and biochemical) have never been compared and validated for the European anchovy Engraulis encrasicolus, the European pilchard Sardina pilchardus and the European sprat Sprattus sprattus. The accuracy of two indirect methods, the morphometric relative condition index Kn and the bioenergetics index determined with the Distell Fish Fatmeter was investigated by comparing with a direct measure of relative lipid content carried out with a thin layer chromatography-flame ionization detector. Estimations from the fatmeter... |
| Tipo: Text |
Palavras-chave: Anchovy; Fatmeter; Lipids; Mediterranean Sea; Sardine; Sprat. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00226/33741/32304.pdf |
| |
|
|
| Chouvelon, Tiphaine; Schaal, G.; Grall, J.; Pernet, Fabrice; Perdriau, M.; A-pernet, E. J.; Le Bris, H.. |
| Anthropogenic activities and land-based inputs into the sea may influence the trophic structure and functioning of coastal and continental shelf ecosystems, despite the numerous opportunities and services the latter offer to humans and wildlife. In addition, hydrological structures and physical dynamics potentially influence the sources of organic matter (e.g., terrestrial versus marine, or fresh material versus detrital material) entering marine food webs. Understanding the significance of the processes that influence marine food webs and ecosystems (e.g., terrestrial inputs, physical dynamics) is crucially important because trophic dynamics are a vital part of ecosystem integrity. This can be achieved by identifying organic matter sources that enter food... |
| Tipo: Text |
|
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00275/38612/37468.pdf |
| |
|
|
| Cochennec-laureau, Nathalie; Baud, Jean-pierre; Pepin, Jean-francois; Benabdelmouna, Abdellah; Soletchnik, Patrick; Lupo, Coralie; Garcia, Celine; Arzul, Isabelle; Boudry, Pierre; Huvet, Arnaud; Pernet, Fabrice; Bachere, Evelyne; Bedier, Edouard; Petton, Bruno; Gaussem, Florian; Stanisiere, Jean-yves; Degremont, Lionel. |
| Depuis 2008, la filière ostréicole française doit faire face à des surmortalités exceptionnelles des naissains d’huîtres creuses, Crassostrea gigas, (huîtres de moins d’un an) comprises entre 60 et 90% dans tous les sites d’élevage. Des cas de mortalité similaires ont été décrits en Irlande, dans les îles anglo-normandes, au Portugal en 2009 et au Royaume-Uni en 2010. Récemment deux épisodes de mortalité, associés à la présence d’un virus OsHV1 μvar (ou un variant très proche) ont été également décrits en Nouvelle Zélande (décembre 2010) et en Australie (janvier 2011) soulignant la vulnérabilité de la filière ostréicole dans le monde. L’objectif de ce document est de faire un point sur les recherches menées depuis l’apparition de ce phénomène et de... |
| Tipo: Text |
|
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00033/14423/11719.pdf |
| |
|
|
| Corporeau, Charlotte; Tamayo, David; Pernet, Fabrice; Quere, Claudie; Madec, Stephanie. |
| Pacific oyster Crassostrea gigas were inoculated with OsHV-1 at low load (control) or high load (challenged) to better understand the pathogenesis of ostreid herpesvirus 1 (OsHV-1 μVar) and to determine which metabolic pathways might be affected during infection. Animals were sampled for proteomic analysis two days post-injection, at the same time as OsHV-1 initiated an intense replication phase in challenged oysters. Twenty-five abundant protein spots that showed a marked change in accumulated levels were identified using a two-dimensional electrophoresis (2-DE) proteomic approach. Overall, these proteins are involved in cytoskeleton organization, protein turnover, induction of stress signals, signalling pathways and energy metabolism. Challenged oysters... |
| Tipo: Text |
Palavras-chave: Marine bivalves; Crassostrea gigas; Herpesvirus; Metabolism; Warburg. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00199/31035/29439.pdf |
| |
|
|
| Couturier, Lydie; Michel, Loic; Amaro, Teresa; Budge, Suzanne M; Da Costa, Elisabete; De Troch, Marleen; Di Dato, Valeria; Fink, Patrick; Giraldo, Carolina; Le Grand, Fabienne; Loaiza, Iván; Mathieu-resuge, Margaux; Nichols, Peter D; Parrish, Christopher C; Sardenne, Fany; Vagner, Marie; Pernet, Fabrice; Soudant, Philippe; Browman, Howard. |
| Determining the lipid content and fatty acid (FA) composition of aquatic organisms has been of major interest in trophic ecology, aquaculture, and nutrition for over half a century. Although protocols for lipid analysis are well-described, their application to aquatic sciences often requires modifications to adapt to field conditions and to sample type. Here, we present the current state of knowledge of methods dedicated to both marine and freshwater lipid analyses, from sampling to data treatment. We review: (i) sample preservation, storage and transport protocols, and their effects on lipids, (ii) lipid extraction, separation of polar and neutral lipids, derivatization, and detection methods, and (iii) available tools for the statistical analysis of FA... |
| Tipo: Text |
Palavras-chave: Aquatic nutrition; Biomarkers; Fatty acids; Marine and freshwater lipids; Methods; Recommended practices. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00651/76279/77252.pdf |
| |
|
|
| Curd, Amelia; Pernet, Fabrice; Corporeau, Charlotte; Delisle, Lizenn; Firth, Louise B.; Nunes, Flavia; Dubois, Stanislas. |
| The honeycomb worm Sabellaria alveolata is capable of building extensive bioconstructions, including what are currently considered Europe’s largest biogenic reefs. The size and volume of these bioconstructions, however, vary greatly, such that not all habitats engineered by S. alveolata may be easily identified as reefs. Given that European environmental legislation protects marine habitats that are classified as “reefs”, it is important to identity a clear set of definition criteria. Furthermore, quantifiable and unequivocal criteria are also needed to evaluate the ecological (health) state of these reefs, in order to best monitor and protect them. Here we propose new terminology to describe the physical appearance of these bioconstructions and attempt to... |
| Tipo: Text |
Palavras-chave: Biochemical indicators; Health; Ecological status; Sabellaria alveolata; Biogcnic reef; Engineer species. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00464/57608/60175.pdf |
| |
|
|
| De Decker, Sophie; Normand, Julien; Saulnier, Denis; Pernet, Fabrice; Castagnet, Sophie; Boudry, Pierre. |
| Several Vibrio species are known to be pathogenic to the Pacific oyster Crassostrea gigas. Survival varies according to pathogen exposure and high mortality events usually occur in summer during gametogenesis. In order to study the effects of gametogenetic status and ploidy (a factor known to affect reproduction allocation in oysters) on vibriosis survival, we conducted two successive experiments. Our results demonstrate that a common bath challenge with pathogenic Vibrio splendidus and Vibrio aestuarianus on a mixture of mature, spawning and non-mature oysters can lead to significant mortality. Previous bath challenges, which were done using only non-mature oysters, had not produced mortality. Immunohisto-chemical analyses showed the affinity of Vibrio... |
| Tipo: Text |
Palavras-chave: Experimental vibriosis; Crassostrea gigas; Vibrio; Survival distribution function; Triploidy; Reproductive effort. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00030/14108/11368.pdf |
| |
|
|
| De Lorgeril, Julien; Escoubas, Jean Michel; Loubiere, Vincent; Pernet, Fabrice; Le Gall, Patrik; Vergnes, Agnes; Aujoulat, Fabien; Jeannot, Jean-luc; Jumas-bilak, Estelle; Got, Patrice; Gueguen, Yannick; Destoumieux-garzon, Delphine; Bachere, Evelyne. |
| Since 2008, juvenile Crassostrea gigas oysters have suffered from massive mortalities in European farming areas. This disease of complex etiology is still incompletely understood. Triggered by an elevated seawater temperature, it has been associated to infections by a herpes virus named OsHV-1 as well as pathogenic vibrios of the Splendidus clade. Ruling out the complexity of the disease, most of our current knowledge has been acquired in controlled experiments. Among the many unsolved questions, it is still ignored what role immunity plays in the capacity oysters have to survive an infectious episode. Here we show that juvenile oysters susceptible to the disease mount an inefficient immune response associated with microbial permissiveness and death. We... |
| Tipo: Text |
Palavras-chave: Host pathogen interaction; Innate immunity; Invertebrate; Mollusk; In situ mortality; Total bacteria; Crassostrea gigas. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00433/54470/55845.pdf |
| |
|
|
| Degremont, Lionel; Maurouard, Elise; Nourry, Max; Seugnet, Jean-luc; Bedier, Edouard; Fleury, Elodie; Langlade, Aime; Pernet, Fabrice; Pouvreau, Stephane; Le Souchu, Pierrick; Normand, Julien; D'Amico, Florence; Rumebe, Myriam; Cantin, Christian; Barret, Jean; Le Gall, Patrik; Baud, Jean-pierre; Cochennec-laureau, Nathalie; Gervasoni, Erika; Pelissier, Pablo. |
| Ce rapport présente la comparaison des mortalités de deux lots de naissains de Crassostrea gigas dans plusieurs bassins conchylicoles et déterminer le gain de survie obtenu par la sélection pour des huîtres diploïdes |
| Tipo: Text |
Palavras-chave: Mortalités; Huître creuse; Crassostrea gigas; Sélection génétique; Naissain. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00065/17644/15180.pdf |
| |
|
|
| Delisle, Lizenn; Fuhrmann, Marine; Quere, Claudie; Pauletto, Marianna; Pichereau, Vianney; Pernet, Fabrice; Corporeau, Charlotte. |
| Voltage-dependent anion channel (VDAC) is a key mitochondrial protein. VDAC drives cellular energy metabolism by controlling the influx and efflux of metabolites and ions through the mitochondrial membrane, playing a role in its permeabilization. This protein exerts a pivotal role during the white spot syndrome virus (WSSV) infection in shrimp, through its involvement in a particular metabolism that plays in favor of the virus, the Warburg effect. The Warburg effect corresponds to an atypical metabolic shift toward an aerobic glycolysis that provides energy for rapid cell division and resistance to apoptosis. In the Pacific oyster Crassostrea gigas, the Warburg effect occurs during infection by Ostreid herpesvirus (OsHV-1). At present, the role of VDAC in... |
| Tipo: Text |
Palavras-chave: Voltage-dependent anion channel; Warburg effect; Crassostrea gigas; Ostreid herpes virus. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00420/53129/54142.pdf |
| |
|
|
| Delisle, Lizenn; Pauletto, Marianna; Vidal-dupiol, Jeremie; Petton, Bruno; Bargelloni, Luca; Montagnani, Caroline; Pernet, Fabrice; Corporeau, Charlotte; Fleury, Elodie. |
| Of all environmental factors, seawater temperature plays a decisive role in triggering marine diseases. Like fever in vertebrates, high seawater temperature could modulate the host response to pathogens in ectothermic animals. In France, massive mortality of Pacific oysters, Crassostrea gigas, caused by the ostreid herpesvirus 1 (OsHV-1) is markedly reduced when temperatures exceed 24°C in the field. In the present study we assess how high temperature influences the host response to the pathogen by comparing transcriptomes (RNA sequencing) during the course of experimental infection at 21°C (reference) and 29°C. We show that high temperature induced host physiological processes that are unfavorable to the viral infection. Temperature influenced the... |
| Tipo: Text |
Palavras-chave: Anti-viral molecular pathway; Host-pathogen interaction; Marine disease; OsHV-1; Resistance; Temperature. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00656/76806/77974.pdf |
| |
|
| |
|
|
| Epelboin, Yanouk; Quere, Claudie; Pernet, Fabrice; Pichereau, Vianney; Corporeau, Charlotte. |
| Here, we assess the physiological effects induced by environmental concentrations of pesticides in Pacific oyster Crassostrea gigas. Oysters were exposed for 14 d to trace levels of metconazole (0.2 and 2 mu g/L), isoproturon (0.1 and 1 mu g/L), or both in a mixture (0.2 and 0.1 mu g/L, respectively). Exposure to trace levels of pesticides had no effect on the filtration rate, growth, and energy reserves of oysters. However, oysters exposed to metconazole and isoproturon showed an overactivation of the sensing-kinase AMP-activated protein kinase alpha (AMPK alpha), a key enzyme involved in energy metabolism and more particularly glycolysis. In the meantime, these exposed oysters showed a decrease in hexokinase and pyruvate kinase activities, whereas 2-DE... |
| Tipo: Text |
|
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00284/39490/44227.pdf |
| |
|
|
| Fleury, Elodie; Barbier, Pierrick; Petton, Bruno; Normand, Julien; Thomas, Yoann; Pouvreau, Stephane; Daigle, Gaétan; Pernet, Fabrice. |
| Diseases pose an ongoing threat to aquaculture, fisheries and conservation of marine species, and determination of risk factors of disease is crucial for management. Our objective was to decipher the effects of host, pathogen and environmental factors on disease-induced mortality of Pacific oysters (Crassostrea gigas) across a latitudinal gradient. We deployed young and adult oysters at 13 sites in France and we monitored survival, pathogens and environmental parameters. The young oysters came from either the wild collection or the hatchery while the adults were from the wild only. We then used Cox regression models to investigate the effect of latitude, site, environmental factors and origin on mortality risk and to extrapolate this mortality risk to the... |
| Tipo: Text |
|
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00627/73915/73251.pdf |
| |
|
|
| Fleury, Elodie; D'Amico, Florence; Annezo, Jean-pierre; Barret, Jean; Blin, Jean Louis; Bouget, Jean-francois; Claude, Serge; Guilpain, Patrice; Grizon, James; Hitier, Benoist; Langlade, Aime; Le Gall, Patrik; Le Souchu, Pierrick; Martin, Anne-genevieve; Mary, Charlotte; Normand, Julien; Parrad, Sophie; Penot, Julia; Pernet, Fabrice; Pien, Sebastien. |
| Depuis 2009, l'Observatoire Conchylicole assure le suivi, sur des sites ateliers disposés sur l'ensemble du littoral français, de lots sentinelles d'huîtres creuses Crassostrea gigas représentatifs de la production française. Leur suivi permet d'acquérir des données nationales de croissance et de mortalité, de traduire la dynamique spatio-temporelle des performances d’élevage et ainsi de participer à la compréhension des phénomènes observés. Pour ce faire, des lots sentinelles d’huîtres correspondant à différentes origines (captage ou écloserie, diploïdes ou triploïdes) et à différents stades d’élevage (naissain ou adultes 18 mois) sont déployés simultanément sur 13 sites ateliers représentant les grandes régions conchylicoles du littoral français. En... |
| Tipo: Text |
|
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00083/19433/17047.pdf |
| |
|
|
| Fuhrmann, Marine; Delisle, Lizenn; Petton, Bruno; Corporeau, Charlotte; Pernet, Fabrice. |
| The Pacific oyster, Crassostrea gigas, is an osmoconforming bivalve exposed to wide salinity fluctuations. The physiological mechanisms used by oysters to cope with salinity stress are energy demanding and may impair other processes, such as defense against pathogens. This oyster species has been experiencing recurrent mortality events caused by the Ostreid herpesvirus 1 (OsHV-1). The objectives of this study were to investigate the effect of salinity (10, 15, 25 and 35‰) on energetic reserves, key enzyme activities and membrane fatty acids, and to identify the metabolic risk factors related to OsHV-1-induced mortality of oysters. Acclimation to low salinity led to increased water content, protein level, and energetic reserves (carbohydrates and... |
| Tipo: Text |
Palavras-chave: Bivalve; Disease; Environment; Metabolism; Mortality risk; Salinity. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00427/53886/54958.pdf |
| |
|
|
| Fuhrmann, Marine; Petton, Bruno; Quillien, Virgile; Faury, Nicole; Morga, Benjamin; Pernet, Fabrice. |
| Mortality of young Pacific oysters Crassostrea gigas associated with the ostreid herpesvirus 1 (OsHV-1) is occurring worldwide. Here, we examined for the first time the effect of salinity on OsHV-1 transmission and disease-related mortality of C. gigas, as well as salinity-related effects on the pathogen itself. To obtain donors for OsHV-1 transmission, we transferred laboratory-raised oysters to an estuary during a disease outbreak and then back to the laboratory. Oysters that tested OsHV-1 positive were placed in seawater tanks (35‰, 21°C). Water from these tanks was used to infect naïve oysters in 2 experimental setups: (1) oysters acclimated or non-acclimated to a salinity of 10, 15, 25 and 35‰ and (2) oysters acclimated to a salinity of 25‰; the... |
| Tipo: Text |
Palavras-chave: Bivalve; Herpesviridae; Infection; Disease transmission; Risk analysis. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00350/46100/45781.pdf |
| |
| Registros recuperados: 76 | |
|
|
|
